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polypeptide(s)  (ImmunoGen Inc)

 
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    Structured Review

    ImmunoGen Inc polypeptide(s)
    Polypeptide(s), supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypeptide(s)/product/ImmunoGen Inc
    Average 90 stars, based on 1 article reviews
    polypeptide(s) - by Bioz Stars, 2026-06
    90/100 stars

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    Image Search Results


    Spore coat expression of SARS-CoV-2 proteins. Panel ( A ) PK120 ( thyA::cotB-RBD Wuh ) and PK122 ( thyA::cotC-HR1-HR2 Wuh ) express the RBD and HR1-HR2 domains of Spike from the Wuhan-Hu-1 (2019-nCoV) variant when probed with anti-S (Wuhan-Hu-1) PAbs. Bands corresponding to CotB-RBD Wuh (62 kDa) and CotC-HR1-HR2 Wuh (40 kDa) are shown. No bands were detectable in spores of the isogenic strain WT which expresses no heterologous antigens. Panel ( B ) PK230 ( thyA::cotB-RBD Omi ) expresses the RBD domain of Omicron (B.1.1.529) fused to CotB (63 kDa) when probed with anti-S (Omicron) PAbs. No bands were detectable in spores of the strain WT.

    Journal: Vaccines

    Article Title: Heterologous Systemic Prime–Intranasal Boosting Using a Spore SARS-CoV-2 Vaccine Confers Mucosal Immunity and Cross-Reactive Antibodies in Mice as well as Protection in Hamsters

    doi: 10.3390/vaccines10111900

    Figure Lengend Snippet: Spore coat expression of SARS-CoV-2 proteins. Panel ( A ) PK120 ( thyA::cotB-RBD Wuh ) and PK122 ( thyA::cotC-HR1-HR2 Wuh ) express the RBD and HR1-HR2 domains of Spike from the Wuhan-Hu-1 (2019-nCoV) variant when probed with anti-S (Wuhan-Hu-1) PAbs. Bands corresponding to CotB-RBD Wuh (62 kDa) and CotC-HR1-HR2 Wuh (40 kDa) are shown. No bands were detectable in spores of the isogenic strain WT which expresses no heterologous antigens. Panel ( B ) PK230 ( thyA::cotB-RBD Omi ) expresses the RBD domain of Omicron (B.1.1.529) fused to CotB (63 kDa) when probed with anti-S (Omicron) PAbs. No bands were detectable in spores of the strain WT.

    Article Snippet: Strains were confirmed by preparing spores, extracting spore coat proteins and probing Western blots with antibodies to the relevant SARS-CoV-2 domains: anti-S Wuh PAbs raised against the entire S Wuh polypeptide (SinoBiological, 40589-T62) and anti-S Omi MAbs that were raised against the RBD of the S Omi (SinoBiological, 40592-MM117).

    Techniques: Expressing, Variant Assay